VASP anticorps (pSer239) (FITC)

N° du produit ABIN126911

L’anticorps Souris Monoclonal anti-VASP a été validé pour IF et FACS. Il convient pour détecter VASP dans des échantillons de Humain et Souris.

Aperçu rapide pour VASP anticorps (pSer239) (FITC) (ABIN126911)

Antigène: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp VASP est conjugé à/à la FITC

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 16C2

Détail du produit anti-VASP anticorps (FITC)

Épitope: pSer239

Specificité: This antibody recognizes VASP only, when Ser239 is phosphorylated, a site preferred by cGMP-dependent protein kinase (PKG) but also used by cAMP-dependent protein kinase (PKA). This antibody does not crossreact with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, The antibody is able to monitor the phosphorylation state of VASP Serine239 as well as PKA activity.

Attributs du produit: Synonyms: Vasodilator-stimulated phosphoprotein

Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography

Immunogène: Synthetic phosphopeptide conjugated to KLHEpitope: Phosphoserine 239 RKVpSKQE

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry. Immunocytochemistry: 1-10 μg/mL (may tolerate 0.5 % formaldehyde fixation).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: 2 x PBS containing 0.09 % Sodium Azide / PEG and Sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Aliquote and freeze (in liquid nitrogen at -20 °C to -80 °C). Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur VASP

Antigène: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Autre désignation: VASP

Sujet: VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling pathways. VASP is expressed in almost all human and animal cell lines, particularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formatSynonyms: Vasodilator-stimulated phosphoprotein

ID gène: 7408

UniProt: P50552

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation